Optical monitoring of microreactor platforms
Microreactors come in many shapes and sizes and allow chemical and biological conversions confined to very small volumes. Current methods for monitoring of these reaction vessels are mostly limited in sensitivity or selectivity or demand extensive procedures such as labeling protocols and/or sampling for off-line analytical determinations. In a recent work we have described the implementation and application of a luminescent oxygen sensor into a micro flow reactor and used it for real-time label-free monitoring of a catalytic oxidation process (Fig. 1). Real-time observation of process parameters on microreactors facilitates the development of on-chip synthesis and modification procedures and can also be very interesting for binding and kinetic studies. Currently we are working on the implementation of biosensors and novel formats, integration procedures, sensors and detection methods.
Publications in this area
(8) S.A. Pfeiffer, S.M. Borisov, S. Nagl,
In-line monitoring of pH and oxygen during enzymatic reactions in off-the-shelf all-glass microreactors using integrated luminescent microsensors, Microchim. Acta, in press
(7) C. Herzog, E. Poehler, A.J. Peretzki, S.M. Borisov, D. Aigner, T. Mayr, S. Nagl,
Continuous on-chip fluorescence labelling, free-flow isoelectric focusing and marker-free isoelectric point determination of proteins and peptides, Lab Chip 2016, 16, 1565-1572
(6) C. Hoera, S. Ohla, Z. Shu, E. Beckert, S. Nagl, D. Belder, An integrated microfluidic chip enabling control and spatially resolved monitoring of temperature in micro flow reactors, Anal. Bioanal. Chem., 2015, 407, 387-396
(5) J. Cao, S. Nagl, E. Kothe, J. M. Köhler, Application of oxygen sensor microparticles for monitoring bacterial growth and characterization of dose/response functions in microfluidic screenings, Microchim. Acta, 2015, 182, 385-394
(4) S. Jezierski, V. Tehsmer, S. Nagl, D. Belder, Integrating microflow reactions with subsequent continuous product separation in a single microfluidic chip, Chem. Commun. 2013, 49, 11644-11646
(3) C. Benz, H. Retzbach, S. Nagl, D. Belder, Protein-protein interaction analysis in single microfluidic droplets using FRET and fluorescence lifetime detection, Lab Chip 2013, 13, 2808-2814
(2) L. Gitlin, C. Höra, R.J. Meier, S. Nagl, D. Belder, Micro flow reactor chips with integrated luminescent chemosensors for spatially resolved on-line chemical reaction monitoring, Lab Chip 2013, 13, 4134-4141
(1) S. Ohla, R. Beyreiss, S. Fritzsche, P. Gläser, S. Nagl, K. Stockhausen, C. Schneider, D. Belder, Monitoring on-Chip Pictet-Spengler Reactions by Integrated Analytical Separation and Label-Free Time-Resolved Fluorescence, Chem. Eur. J. 2012, 18, 1240-1246
